Witryna31 paź 2009 · Organotypic epithelial raft cultures accurately reproduce the process of epithelial differentiation in vitro and can be prepared from normal keratinocytes, explanted epithelial tissue, or established cell lines. This culture system permits cells to proliferate and fully differentiate at the air-liquid interface on a dermal-equivalent support. Witryna20 sty 2024 · Abstract. Organ, histotypic, and organotypic culture using mammalian three-dimensional (3D) cell culture technologies are the primary focus of this chapter. The major purpose of organ, histotypic, and organotypic culture is to create in vitro models comparable to the in vivo environment which may replace old, damaged/injured, or …
Production of infectious bovine papillomavirus from cloned viral ... - PNAS
WitrynaThe organotypic (raft) culture system has been used to develop an in vitro system that is capable of reproducing the entire human papillomavirus (HPV) life cycle, including virion morphogenesis. This system utilizes HPV-containing cell lines that are either derived from biopsies or created by the transfection of keratinocytes with HPV … WitrynaOrganotypic raft cultures show epithelium-specific, harmful effects of topical drugs, provided the compounds are supplied onto the culture surface and not into the culture … gigi\u0027s cupcakes lafayette in
Native Human Papillomavirus Production, Quantification, and …
WitrynaAbstract. We have developed an in vitro organotypic (raft) culture system capable of reproducing the differentiation-dependent replication cycle of human papillomavirus. A … Witryna11 sty 2024 · Introduction of the S23A mutation into the HPV16 genome results in a loss of E2 expression and viral genome integration during organotypic rafting. Co-culture of N/Tert-1+E2-S23A cells with J2 fibroblasts results in E2-S23A degradation via the proteasome, wild-type E2 is not degraded. WitrynaAdditionally, one of the selected compounds, the acridine derivative C8, demonstrated a significant antiviral effect in HPV18-infected organotypic raft cultures. Altogether, these results indicate that targeting HPV G-quadruplexes may be an alternative route for the development of novel antiviral therapies. gigi\u0027s daughter birthday