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Organotypic raft

Witryna31 paź 2009 · Organotypic epithelial raft cultures accurately reproduce the process of epithelial differentiation in vitro and can be prepared from normal keratinocytes, explanted epithelial tissue, or established cell lines. This culture system permits cells to proliferate and fully differentiate at the air-liquid interface on a dermal-equivalent support. Witryna20 sty 2024 · Abstract. Organ, histotypic, and organotypic culture using mammalian three-dimensional (3D) cell culture technologies are the primary focus of this chapter. The major purpose of organ, histotypic, and organotypic culture is to create in vitro models comparable to the in vivo environment which may replace old, damaged/injured, or …

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WitrynaThe organotypic (raft) culture system has been used to develop an in vitro system that is capable of reproducing the entire human papillomavirus (HPV) life cycle, including virion morphogenesis. This system utilizes HPV-containing cell lines that are either derived from biopsies or created by the transfection of keratinocytes with HPV … WitrynaOrganotypic raft cultures show epithelium-specific, harmful effects of topical drugs, provided the compounds are supplied onto the culture surface and not into the culture … gigi\u0027s cupcakes lafayette in https://fixmycontrols.com

Native Human Papillomavirus Production, Quantification, and …

WitrynaAbstract. We have developed an in vitro organotypic (raft) culture system capable of reproducing the differentiation-dependent replication cycle of human papillomavirus. A … Witryna11 sty 2024 · Introduction of the S23A mutation into the HPV16 genome results in a loss of E2 expression and viral genome integration during organotypic rafting. Co-culture of N/Tert-1+E2-S23A cells with J2 fibroblasts results in E2-S23A degradation via the proteasome, wild-type E2 is not degraded. WitrynaAdditionally, one of the selected compounds, the acridine derivative C8, demonstrated a significant antiviral effect in HPV18-infected organotypic raft cultures. Altogether, these results indicate that targeting HPV G-quadruplexes may be an alternative route for the development of novel antiviral therapies. gigi\u0027s daughter birthday

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Category:Differentiation of HPV-Containing Cells Using Organotypic “Raft ...

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Organotypic raft

Using Organotypic (Raft) Epithelial Tissue Cultures for the ...

Witryna12 paź 2014 · Utilization of an organotypic raft culture system allows researchers to investigate all aspects of the viral life cycle, including viral DNA amplification, late gene expression, virion morphogenesis, and infectivity. This chapter will explain three techniques. First, we will show how to set up and produce HPV from the organotypic … WitrynaArgonaute-2 protein (Ago2), a major component of RNA-induced silencing complex (RISC), has been viewed as a cytoplasmic protein. In this study, we demonstrated by immunofluorescence confocal microscopy that Ago2 is distributed mainly as a nuclear protein in primary human foreskin keratinocytes in monolayer cultures and their …

Organotypic raft

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Witryna(B) Sections of organotypic raft cultures generated from TFAP2A-, TFAP2C- or TP63-depleted HFKs stained for haematoxylin & Eosin (H&E) and indirect immunofluorescent staining of early [keratins 1 ... Witryna17 lis 2008 · Organotypic epithelial raft culture. Organotypic rafts were generated as described in (Nakahara et al., 2005). Briefly, a total of 1×10 6 human keratinocytes was plated on a collagen matrix with embedded feeder fibroblasts. Exposure to the liquid-air interface resulted in the generation of stratified epithelium with differentiation …

WitrynaA) Outline of method for preparing organotypic raft cultures from primary keratinocytes or epithelial cell lines. B) Hematoxylin and eosin staining of organotypic raft cultures generated from human foreskin keratinocytes stably maintaining HPV-31 (HFK-31) episomes, or from normal human foreskin keratinocytes (HFK). Witryna12 paź 2014 · Organotypic raft cultures. (a) Cotransfect plasmids into PHKs on plate.(b) Select for transfected cells by drug resistance.The details are described in Fig. 2.(c) Transfer cells onto a collagen bed and culture in K-SFM for 2 days.(d) Lift the assembly to the air: medium interface.(e) Culture for 10 days or more, whereupon cells …

WitrynaCurrent techniques allow for the establishment of recombinant wild-type or mutant human papillomavirus (HPV) genomes in transfected early-passage human foreskin … Witryna3.3 Organotypic (Raft) Culturing Technique for Inducing Terminal Differentiation of NIKS. In this section, a detailed methodology for producing stratified squamous epithelia using an organotypic culturing approach is described. This is commonly referred to as “raft” culture because one “floats” the keratinocyte cell culture at the air ...

Witryna1 sie 2014 · The apparent floating nature of the cell-matrix in this method led to the nickname "raft" cultures. The general technique can be applied to normal low …

Witryna22 lut 2012 · The development of organotypic epithelial raft cultures has provided researchers with an efficient in vitro system that faithfully recapitulates epithelial … gigi\u0027s cupcakes polaris ohioWitrynaWe have developed an in vitro organotypic (raft) culture system capable of reproducing the differentiation-dependent replication cycle of human papillomavirus. A dermal equivalent is made from a mixture of type I collagen and fibroblasts. Cells of a line derived from a cervical intraepithelial neoplasia type 1 (CIN-1) are placed on top of … gigi\u0027s cupcakes oklahoma cityWitryna1 mar 2024 · When grown as organotypic raft cultures, HPV16-infected cells expressed late E1^E4 and L1 proteins and replication foci were detected, suggesting that they … gigi\u0027s cupcakes oak creek wiWitryna6 sty 2024 · For organotypic raft and tissue sections, slides were deparaffinized, and antigen retrieval was performed in 10 mM sodium citrate pH 7 for 15 min at 110 °C in a decloacker chamber (Biocare, Pacheco, CA, USA). For 2D coverslips, permeabilization was performed with 0.2% Triton X-100 for 3 min at room temperature (RT). … gigi\u0027s day off photo with monkeyWitrynaContributed by Matt Lewis . Organotypic raft culture of primary human keratinocytes. Trypsinize the PHKs off the flask and resuspend in (say) 2ml … gigi\u0027s cupcakes menu with pricesWitryna21 lut 2024 · Organotypic epithelial raft cultures (OERCs) are 3D in vitro culture systems that permit terminal differentiation of keratinocytes—the major cell type of the … ft george promotional codeWitryna19 sty 2024 · In this study, we recapitulate the histopathology of human MCC tumors in vitro using an organotypic (raft) culture system that is traditionally used to recapitulate the dermal and epidermal ... gigi\u0027s cupcakes raleigh nc