Organotypic hippocampal slice culture

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August undefined, 2024

Witryna18 sty 2024 · Organotypic hippocampal slice culture (OHSC) Rat organotypic hippocampal-entorhinal cortical slice cultures were prepared as described previously [48, 49].Briefly, neonates rat pups at postnatal day 7 (P7) were decapitated, brain removed and hippocampal-entorhinal complex dissected in Gey’s buffer (Sigma … Witryna3 lut 2011 · Organotypic hippocampal slice cultures J Vis Exp. 2011 Feb 3;(48):2462. doi: 10.3791/2462 . ... Organotypic hippocampal cultures offer the possibility for …

Iron toxicity in organotypic cultures of hippocampal slices: role of ...

WitrynaAbstract. Slices of developing brain tissue can be grown for several weeks as so-called organotypic slice cultures. Here we summarize and review studies using … Witryna7 wrz 2006 · Organotypic slice cultures from ∼ 1-week-old mouse hippocampus appear to reproduce most anatomical and functional properties of the corresponding hippocampal circuits in vivo for at least 6 ... minecraft pranks prestonplayz

Protocol to generate murine organotypic brain cultures for drug ...

WitrynaSlices of developing brain tissue can be grown for several weeks as socalled organotypic slice cultures. Here we summarize and review studies using hippocampal slice cultures to investigate mechanisms and treatment strategies for the neurodegenerative disorders like stroke (cerebral ischemia), Alzheimers disease (AD) … WitrynaThis protocol describes a method for making and culturing rat hippocampal organotypic slices on membrane inserts. Supplementary videos are included to demonstrate … WitrynaIn this section, cultured brain slices or organotypic cultures are subjected to BLI and fixation. 17. Add 20 μL of D-luciferin (15 mg/mL) per mL of SCM in the 12-well plate, gently shake the plate to mix, and incubate 5 min. ... Preparation of organotypic hippocampal slice cultures: interface method. Nat. Protoc., 1 (2006), pp. 1439 … morrowind no sound

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Comparison of neuroprotective effects of erythropoietin (EPO) and ...

WitrynaOrganotypic hippocampal slice cultures (OHSCs) have been used as a powerful ex vivo model for decades. They have been used successfully in studies of neuronal … WitrynaThis protocol details a method to establish organotypic slice cultures from mouse hippocampus, which can be maintained for several months. The cultures are based on the interface method, which does not require special equipment, is easy to execute and yields slice cultures that can be imaged repeatedly – from when they are isolated at … minecraft prank mod 1.12.2 curseforgeWitryna1 sty 2024 · Organotypic hippocampal brain slice cultures. (A, B) The rocking plate method of brain slice culture. Slice is placed into a poly-d-lysine (PDL) coated 35 … minecraft pranks 2022

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Organotypic hippocampal slice culture

WitrynaWe have provided a detailed protocol for the preparation of interface hippocampal slice cultures from young mice or rats and have included modifications of the protocol necessary for culturing electrophysiologically viable slices from older animals. In addition to providing key points for successful measurements of synaptic function using ... Witryna1 paź 2015 · In organotypic hippocampal slice cultures it was shown (Kreutz et al., 2011) that ganglioside GM1 exhibited a neuroprotective activity on beta-amyloid …

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Witryna3 lut 2011 · The organotypic hippocampal slice culture (OHSC) is a useful model for studying the neurodegeneration process, but there are still many protocol differences. … WitrynaSummaryAutomatic TranslationFebruary 3rd, 2011. We describe a method to prepare organotypic hippocampal slices that can be easily adapted to other brain regions. …

Witryna12 kwi 2024 · Organotypic tissue cultures are characterized by their laminar organization of hippocampal pathways and cortico-hippocampal projections in steady-state conditions (Frotscher and Heimrich, 1993; Heimrich and Frotscher, 1993) that resemble the in vivo architecture of synapses at different levels (Maus et al., 2024). WitrynaLein PJ, Barnhart CD, Pessah IN (2011) Acute hippocampal slice preparation and hippocampal slice cultures. Methods Mol Biol 758:115–134 Gogolla N, Galimberti I, DePaola V, Caroni P (2006) Preparation of organotypic hippocampal slice cultures for long-term live imaging. Nat Protoc 1:1165–1171

Witryna2 gru 2024 · Organotypic slice cultures of brain or spinal cord have been a longstanding tool in neuroscience research but their utility for understanding … Witryna1 sty 2024 · Organotypic hippocampal brain slice cultures. (A, B) The rocking plate method of brain slice culture. Slice is placed into a poly-d-lysine (PDL) coated 35-mm dish, or into one of the PDL-coated wells of a 6-well tissue culture plate. Slice placement is slightly off the rocking axis, to facilitate movement into and out of culture medium (B).

Witrynaorganotypic cultures of hippocampal slices to study potential mechanisms involved in iron-induced neuronal damage. Exposure of mature hippocampal slices to ferrous sulfate resulted in concentration- and time-dependent cell death. After iron treatment, markers of ROS formation and lipid peroxidation, i.e. intensity of dichloroﬂuorescein …

Witryna6 kwi 2024 · Organotypic hippocampal slices were prepared from Wistar rats of both sexes (Jackson-No. 031628) at post-natal days 5–7 as previously described 59. In brief, dissected hippocampi were cut into ... morrowind npc commandsWitrynaComparison of neuroprotective effects of erythropoietin (EPO) and carbamylerythropoietin (CEPO) against ischemia-like oxygen–glucose deprivation (OGD) and NMDA excitotoxicity in mouse hippocampal slice cultures morrowind not recognizing keyboardsWitryna15 maj 2024 · Introduction. Organotypic hippocampal slice cultures provide a convenient way of maintaining hippocampal tissue in culture conditions in vitro long-term. Hippocampal architectural morphology and perforant pathway connections are retained from their organisations in the donor brain, while allowing ease of … minecraft pranks easyWitryna12 kwi 2024 · Organotypic tissue cultures are characterized by their laminar organization of hippocampal pathways and cortico-hippocampal projections in … minecraft pranks no modWitryna2 lis 2006 · Noraberg, J. et al. Organotypic hippocampal slice cultures for studies of brain damage, neuroprotection and neurorepair. Curr. Drug Targets CNS Neurol. … morrowind npc blocking doorWitryna21 mar 2024 · This paper examines the effects of blast-induced traumatic brain injury (bTBI) on long-term potentiation (LTP) and microglial … morrowind npc force wearWitryna27 sie 2024 · In organotypic hippocampal slice cultures (OHSC), the morphological and functional characteristics of both neurons and glial cells are well preserved. This model is suitable for addressing different research questions that involve studies on neuroprotection, electrophysiological experiments on neurons, neuronal networks or … morrowind npc names