WebFeb 28, 2014 · Changes in Δψ m, visualized by change in the fluorescence of JC-1, were investigated within one hour after reconstitution of oxygen and glucose supply ... Stained, polarized mitochondria were detected with fluorescence settings for Cy3 (excitation/emission = 550/570 nm, EV = 50 msec). Loss of mitochondrial integrity was ... Web2 days ago · A fluorescence assay was developed based on aggregation-induced emission for circulating tumor (ctDNA) rapid detection. AIE-based probe (TPE-DNA) …

Why are cy3 and cy5 dyes used as a FRET fluorophore pair?

WebTwo-photon absorption and emission spectra for fluorophores relevant in cell imaging were measured using a 45 fs Ti:sapphire laser, a continuously tuneable optical parametric amplifier for the excitation range 580-1150 nm and an optical multichannel analyser. The measurements included DNA stains, fluorescent dyes coupled to antibodies as well ... WebThe fluorescence from Cy5 for all the samples is fairly (at fixed target concentration) the percent hybridization should constant indicating the probe coverage is nominally constant. increase monotonically with the time the chip is exposed to Consistent with the C measurement, the fluorescence from Cy3 due to binding increases monotonically. bwst cam for a gm 400 engine

Cy3 dye Thermo Fisher Scientific - IN

WebCy™3 can be excited to about 50% of maximum with an argon laser (514 nm or 528 nm lines), or to about 75% of maximum with a helium/neon laser (543 nm line) or mercury lamp (546 nm line). Cy™3 can be used with … WebAug 12, 2008 · Fluorescence emission spectra were recorded at 4°C by using an SLM-Aminco 8100 fluorimeter in 90 mM Tris-borate (pH 8.3). ... Up to 100 images of the … WebNIR fluorescence EEM spectroscopy (41 excitation wavelengths ranging from 550 to 950 nm in 10-nm increments; fluorescence emission from 570 to 1000 nm at 10-nm intervals) can be acquired from ... cff9